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Towards Unbiased DBS Analysis

Summary A new technology for automated analysis of DBS samples has been investigated and report- ed previously [3]. This is based on patented flow-through desorption of the blood spot, which can be coupled online to MS/MS analysis via a disposable SPE cartridge for sample clean- up and separation. Here we report how a DBS Autosampler can overcome critica! issues in DBS analysis. The multi-dispenser showed that it can be conveniently handled to sample blood from a finger prick and to dispense 5-I aliquots after- wards onto DBS cards. Touching the DBS card does not result in any analytica! issues; in con- trast it helps to keep the spot diameter small. The entire bloodspot is analyzed so that no sam- ple is wasted. Sample clean-up by online SPE re- duces MS matrix effects to an acceptable level. By using temperature-enhanced desorption con- ditions recoveries close to 100% are attained independent of the blood Ht level. FTD at high temperatures combined with online SPE-MS/MS of full blood spots overcomes the effect of Ht on recovery in DBS analysis. This micro-sampling concept therefore enables a robust and reliable quantitative DBS analysis. In addition, the com- pletely automated workflow omits the cumber- some and error prone practice of punching-out discs trom
Category 2. Symbiosis Application Notes
These are application notes that have been made in our application lab. They were based on customer demo's and customer method development requests. They include sorbent screening, a calibration curve and are optimized for carry over. They are not always (completely) validated
Matrix Blood
Market DBS Clinical
Compounds Chlortalidone, Hydrochlorothiazide, Acebutolol , Ha peridol, Verapamil, Propranolol
Compound Group
Sample_volume 5-I
Contact Christel Hempen, Lena Knegt, Bert Ooms
Account Spark Holland
Date 4-8-2015
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