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Title	High sensitivity assays using online SPE-LC-MS/MS - How low can you go
Summary	Online SPE – No concentration of sample by the use of temperature or pressure (Peptides are generally very susceptible to adsorption problems) – Sample pre-treatment protein precipitation or offline SPE – Sample extract diluted with water and surfactant to adjust polarity – On-line SPE using symbiosis (To aid sample concentration and further sample clean-up) – Allows higher sample loading – Analytical column with reverse phase gradient – This allows linear increase in sensitivity when sample volume is increased
Category	2. Symbiosis Application Notes These are application notes that have been made in our application lab. They were based on customer demo's and customer method development requests. They include sorbent screening, a calibration curve and are optimized for carry over. They are not always (completely) validated
Matrix	Plasma Serum
Market	Clinical, CRO
Cartridge	cartridges C2
Detection
Compounds	Peptides
Compound Group	Peptides
Sample_volume	100 µL
Contact	Mohammed Abrar
Account	Unilabs York Bioanalytical solutions, York, UK
Published	poster
Date	4-8-2015
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