Detailed information | |
Title |
High sensitivity assays using online SPE-LC-MS/MS - How low can you go |
Summary | Online SPE – No concentration of sample by the use of temperature or pressure (Peptides are generally very susceptible to adsorption problems) – Sample pre-treatment protein precipitation or offline SPE – Sample extract diluted with water and surfactant to adjust polarity – On-line SPE using symbiosis (To aid sample concentration and further sample clean-up) – Allows higher sample loading – Analytical column with reverse phase gradient – This allows linear increase in sensitivity when sample volume is increased |
Category | 2. Symbiosis Application Notes These are application notes that have been made in our application lab. They were based on customer demo's and customer method development requests. They include sorbent screening, a calibration curve and are optimized for carry over. They are not always (completely) validated |
Matrix | Plasma Serum |
Market | Clinical, CRO |
Cartridge | cartridges C2 |
Detection | |
Compounds | Peptides |
Compound Group | Peptides |
Sample_volume | 100 µL |
Contact | Mohammed Abrar |
Account | Unilabs York Bioanalytical solutions, York, UK |
Published | poster |
Date | 4-8-2015 |
Read PDF document | |
Remarks | |
Users | |
Status |