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High sensitivity assays using online SPE-LC-MS/MS - How low can you go

Summary Online SPE No concentration of sample by the use of temperature or pressure (Peptides are generally very susceptible to adsorption problems) Sample pre-treatment protein precipitation or offline SPE Sample extract diluted with water and surfactant to adjust polarity On-line SPE using symbiosis (To aid sample concentration and further sample clean-up) Allows higher sample loading Analytical column with reverse phase gradient This allows linear increase in sensitivity when sample volume is increased
Category 2. Symbiosis Application Notes
These are application notes that have been made in our application lab. They were based on customer demo's and customer method development requests. They include sorbent screening, a calibration curve and are optimized for carry over. They are not always (completely) validated
Matrix Plasma Serum
Market Clinical, CRO
Cartridge cartridges C2
Compounds Peptides
Compound Group Peptides
Sample_volume 100 L
Contact Mohammed Abrar
Account Unilabs York Bioanalytical solutions, York, UK
Published poster
Date 4-8-2015
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