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On-Line SPE LC-MS/MS Drug Screening in Serum on a Hybrid Quadrupole/ Linear Ion Trap Instrument

Summary Comprehensive screening for the detection of drugs and toxic compounds in biological samples is an important function of toxicological analysis. As the demand to monitor the ever increasing number of drugs continues to rise, so too does the need to detect and quantify these compounds in a simple, single and automated run; providing a fast turn- around time for the results. This presentation describes the rapid cleanup of serum samples and detection of over 100 drugs using On-line SPE LC-MS/MS screening and confirmation. The LC-MS/MS was operated in Multiple Reaction Mode (MRM) for detection. Dependent MS/MS spectra were acquired in the Enhanced Product Ion (EPI) mode after being triggered from a Scheduled MRM™ Pro Algorithm Information Dependent Acquisition (IDA) survey scan. Combining MRM and product ion spectral acquisition allows for compound identification with highest confidence based on mass spectral library matching. The automated workflow monitors large panels of analytes; detecting and quantifying these compounds in a single run.
Category 2. Symbiosis Application Notes
These are application notes that have been made in our application lab. They were based on customer demo's and customer method development requests. They include sorbent screening, a calibration curve and are optimized for carry over. They are not always (completely) validated
Matrix Serum
Market Toxicology
Cartridge HySphere Resin GP
Detection AB SCIEX QTRAP® 4500 LC/MS/MS
Compounds unknown screening
Compound Group unknown screening
Sample_volume 10 µL
Contact Adrian M. Taylor, Peter Ringeling, Martin Sibum, Stefan Sturm, André Scholer, Roswitha Skendaj
Account AB SCIEX, 71 Four Valley Drive Concord, Ontario, L4K 4V8 Canada; Spark Holland BV, P.O. Box 388, 7800 AJ Emmen, The Netherlands; University Hospital Basel, 4031 Basel, Switzerland.
Published poster
Date 4-8-2015
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