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Quantitatitive Analysis of Phosphorothioate Oligonucleotide in Human Plasma Using LC-MS/MS with On-Line Extraction

Summary Oligonucleotide therapeutics are gaining renewed confidence as a new class of drugs. Many bioanalytical methods for oligonucleotides have been developed to keep up with the pace of drug development needs. LC-MS/MS is one of the most popular techniques due to its unprecedented specificity; however, the sensitivity for most LC-MS/MS assays is still not as good as corresponding ligand binding assays for some large oligonucleotide therapeutics. To improve sensitivity and throughput, we have successfully developed a fully automated online SPE integrated. A highly sensitive and fully automated online SPE-LC-MS/MS method was developed to quantify an 18-mer phosphorothioate DNA oligonucleotide in human plasma.
Category 2. Symbiosis Application Notes
These are application notes that have been made in our application lab. They were based on customer demo's and customer method development requests. They include sorbent screening, a calibration curve and are optimized for carry over. They are not always (completely) validated
Matrix Plasma, Human plasma
Market CRO
Cartridge Spark Holland HySphere C18HD
Detection ABI Sciex API 5000
Compounds Oligonucleotide, phosphorothioate DNA oligonucleotide
Compound Group Oligonucleotide
Sample_volume 100 L
Contact Laixin Wang, Sherry Liu, Qiuying Zhu, Scott Reuschel and Min Meng
Account Tandem Labs
Published poster
Date 4-8-2015
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