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Fully Automated Determination in the Low Nanogram per Liter Level of Different Classes of Drugs of Abuse in Sewage Water by On-Line Solid-Phase Extraction-Liquid Chromatography-Electrospray-Tandem Mass Spectrometry

Summary The present work describes the first fully automated method, based on on-line solid-phase extraction (SPE)-liquid chromatography-electrospray-tandem mass spectrometry, developed for the determination of drugs of abuse (17 compounds and metabolites belonging to the classes of amphetaminics, cannabinoids, cocainics, opi­ates, and lysergics) in sewage waters. On-line SPE is performed by passing 5 mL of the water sample through a PLRP-s cartridge for analytes measured in positive ionization mode (all but cannabinoids) and through an Oasis HLB cartridge for analytes measured in negative ionization mode (cannabinoids). For unequivocal identi­fication and confirmation two selected reaction monitoring transitions are registered per compound, thus achieving the four identification points requested by the European Union for banned substances. Quantitation is performed by the internal standard method, indispensable to correct for matrix effects. The main advantages of the method developed are high sensitivity (limits of determination between 0.69 and 5.97 ng/L), selectivity and reliability of results, minimum sample manipulation, full automa­tion, and fairly high throughput (analysis time per sample is 2 x 35 min). As a part of the validation procedure, the method developed has been applied to the analysis of various influent and effluent samples from four Spanish sewage treatment plants.
Category 3. Symbiosis User Application
These are applications that have been made by Symbiosis customers. They are validated and have been published.
Matrix Water, Sewage Water
Market Environmental
Cartridge PLRP-S and Oasis HLB
Detection MS
Compounds Cocaine, Benzoylecgonine, Cocaethylene, amphetamine, MDMA, Ecstacy, Pseudoephedrine, Ephedrine HCl, Ephedrine, Metamphetamine,Heroine, Morphine, 6-am, 6-acetylmorphine, THC, Nor-THC, OH-THC, LSD, Nor-LSD, O-H-LSD
Compound Group Cocainics, Amphetamine, Opiates, Cannabinoids, LSD
Sample_volume 5 mL
Contact Cristina Postigo, Maria J. Lopez de Alda, Damia Barcelo
Account Department of environmental chemistry, IIQAB-CSIC, CSIC
Published Analytical Chemistry, Vol. 80, No. 9, May 1, 2008
Date 23-5-2008
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Remarks Detection limits in sewage water are around 1 to 5 ng/mL
PLRP-s is used for ESI positive mode compounds. HLB is used for cannabinoids in ESI negative mode.
Users CSIC