Hyphenation and automation of large-volume online-SPE with liquid chromatography mass spectrometry
|Summary||Solid Phase Extraction (SPE) is a technique usually required to achieve an acceptable limit of detection for target analysis in environmental samples. A severe drawback of offline SPE is the time consuming sample preparation. Online SPE circumvents this and enables complete automating of the SPE-LC-MS/MS workflow. Innovative software algorithms help to perform SPE in parallel and can thus reduce the overall cycle time for sample preparation and analysis. For the highest degree of flexibility, the new autosampler platform in combination with an automated cartridge exchanger was used. Therefore, the injection volume can be varied from 10 µL up to 10 mL.|
|Category||2. Symbiosis Application Notes
These are application notes that have been made in our application lab. They were based on customer demo's and customer method development requests. They include sorbent screening, a calibration curve and are optimized for carry over. They are not always (completely) validated
|Compounds||Sotalol, Clarithromycin, Benzotriazol, Sucralose, Ciprofloxacin Ibuprofen, Iomeprol, Ibuprofen, Iomeprol, Benzotriazol and Sucralose|
|Contact||Andreas Bruchmann T. Teutenberg, J. Tuerk, C. vom Eyser, S. Wiese, C. Portner Germany, A. Mohren|
|Account||Axel Semrau GmbH & Co.KG, Stefansbecke 42, 45549 Sprockhoevel, Germany, (Institut für Energie- und Umwelttechnik e. V., IUTA Institute of Energy and Environmental Technology), Bliersheimer Str. 58-60, 47229 Duisburg, Universität Duisburg-Essen, Universitaetsstr. 2, 45141 Essen, Germany|
|Read PDF document|
|Remarks||For sample enrichment C18 phase is not suitable for polar and moderately polar analytes.
Polymeric materials like Resin SH and GP very suitable for enrichment of polar and non polar analytes.
In order to obtain small peak widths small particle packed SPE cartridges (< 5 µm) are preferred.
Innovative software algorithm (Chronos) is a useful tool for interlacing and automation of complete workflow.