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A rapid and sensitive LC-MS/MS method for the simultaneous analysis of testosterone, androstenedione, 17 OHP and DHEAS

Summary Measurement of serum testosterone (T) is necessary for the investigation of androgen disorders in males and females. Measurement of other androgens such as DHEAS and androstenedione (A4) may also be important in the investigation of PCOS and 17 hydroxyprogesterone (17OHP) is useful to differentiate late onset CAH in patients with raised A4. Although it would be advantageous to measure all four steroids simultaneously this is not always easy because of their differing extraction requirements. We describe a simple method for the simultaneous measurement of T, A4, 17OHP and DHEAS using LC-MS/MS after on line sample preparation
Category 2. Symbiosis Application Notes
These are application notes that have been made in our application lab. They were based on customer demo's and customer method development requests. They include sorbent screening, a calibration curve and are optimized for carry over. They are not always (completely) validated
Matrix Serum
Market OSM Clinical
Compounds testosterone, androstenedione, 17 OHP and DHEAS
Compound Group androgens, testosterone
Contact B G Keevil, L J Owen
Account Dept. Clinical Biochemistry, University Hospital of South Manchester Manchester University . United Kingdom
Published poster
Date 4-8-2015
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Status published