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Screening of drugs and toxic compounds in human whole blood using online solid-phase extraction and high performance liquid chromatography time-of-flight mass spectrometry

Summary A novel method used for the screening of 151 drugs and toxic compounds in human whole blood by online solid-phase extraction coupled to liquid chromatography- time-of-flight mass spectrometer (LC-TOF-MSE) has been developed and validated. The sample extraction was performed with online solid- phase extraction which can be fully automated. TOF-MSE screening for 151 drugs and toxic compounds was performed in a full-scan (m/z 50-800) mode using an MSE acquisition of molecular ions and fragment ions data at two collision energies (one was 6eV and another one was in the range of 5-45eV). Total chromatographic run time was 26 min. The compounds were identified based on retention times and exact mass of molecular ions and fragment ions. The limit of detections by online ranged from 1 to 100 ng/mL and the recovery of the method ranged from 6.3% to 163.5%. This method is proved to be a valuable screening method allowing fast and specific identification of drugs in human whole blood.
Category 3. Symbiosis User Application
These are applications that have been made by Symbiosis customers. They are validated and have been published.
Matrix Blood, Whole blood
Market Clinical
Cartridge HySphere Resin GP
Detection Waters Xevo QTOF mass spectrometer
Compounds ephedrine hydrochloride clonidine hydrochloride Raceanisodamine norketamine.HCl norfentanyl oxalate Dylox Acetylcodeine methylcantharidinimide pethidine hydrochloride Papaverine hydrochloride nefopam hydrochloride dextromethorphan hydrobromide Diltiazem hydrochloride Pirimicarb Carbamazepine Imipramine hydrochloride benzhexol hydrochloride clonazepam Trizolam Fluphenazine Hydrochloride Warfarine 2-amino-2'-chloro-5-nitrobenzophen Fenthion Oxymorphone cathine hydrochloride pseudoephedrine hydrochloride naloxone hydrochloride amfetamine sulfate caffeine Hydrocodone PMA.HCL BDB.HCL MBDB.HCL tramadol hydrochloride methylphenidate Dimethoate Acetamiprid 7-aminoflunitrazepam Noscapine fentanyl citras diphenhydramine hydrochloride doxepin hydrochloride Mesaconitine cyproheptadine hydrochloride Verapamil Oxazepam methadone hydrochloride Carbaryl Methaqualone Demethyldiazepam Methyllorazepam Prometryne Alachlor Tetramethrin methylecgonine hydrochloride methcathinone aminorex MDA pemoline thiabendazole benzoylecgonine chlordimeform mexiletine Hydrochloride dihydroetorphine Hydrochloride melatonin fenfluramine hydrochloride clozapine bromazepam haloperidol a-Hydroxyalprazolam betamethasone propoxur alprazolam isoproturon temazepam diazepam acetochlor Hydromorphone Cathinone methylephedrine hydrochloride BullatineA Naltrexone hydrochloride Ethylamphetamine linocaine hydrochloride 4-Phenylbutylamine lofexidine Hydrochloride thebaine Inidacloprid cocaine hydrochliride scopolamine butyl bromide phencycliding Chlordiazepoxide midazolam maleate Paroxetine maleate Hydroxyzine dihydrochloride aconitine carbofuran Lorazepam Chlorpromazine metalaxyl Clobazam Dexamethasone Acetate Paclobutrazol Rotenone Morphine Salbutamol Sulpiride Norcodeine Norcodeine codeine phosphate scopolamine hydrobromide O6-monoacetylmorphine methamphetamine hydrochloride PMMA Methyldiethanolamine MDEA Antipyrine clenbuterol norcocaine zolpidem tetrahydropalmatine Phosphamidon Propafenone Maprotiline Hypaconitine Nitrazepam chlortoluron 2-hydroxyethyl flurazepam pyrimethanil linuron Prazepam Cimetidine dihydrocodeine tartrate Levamisole Oxycodone MDMA Strychnine Brucine ketamine hydrochloride Pheniramine prazosin hydrochloride Protopine Lappaconitine Flurazepam buprenorphine hydrochloride Dexamethasone amitriptyline hydrochloride Estazolam fluoxetine Diuron Isoprocarb Diphenoxylate Nimodipine Parathion
Compound Group Toxic compounds
Sample_volume 50 ÁL
Contact Xiaomei Teng, Yurong Zhang, Rong Wang, Xin Wang, Chen Liang, Yulan Rao, Yuan Lic Tao Sun
Account Shanghai Institute of Forensic Science, Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China b Department of Forensic Medicine (Center of Forensic Science), School of Basic Medical Sciences, Fudan University, Shanghai 200032, China c Esensing Analytical Technology, Shanghai 200336, China
Published not yet
Date 4-8-2015
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